Primary study on estrogen receptor β1 impeding epithelial-mesenchymal transition of breast cancer MDA-MB-231 cells

doi:10.3877/cma. j. issn.1674-0807.2012.04.003

Abstract

Abstract:

Objective

To explore the effect of exogenous ERβ1 gene on the expression of E-cadherin and vimentin by transfecting recombinant eukaryotic expressing vector containing ERβ1 cDNA into human breast cancer MDA-MB-231 cells, and to investigate the biological role of ERβ1 in epithelial-mesenchymal transition of breast cancer cells.

Methods

Recombinant eukaryotic expressing vector containing ERβ1 cDNA was transfected into human breast cancer MDA-MB-231 cells. The mRNA and protein expression levels of ERβ1, E-cadherin and vimentin were tested by real-time polymerase chain reaction and Western blot, respectively. The cell growth curve showed the change of proliferation ability in MDA-MB-231. All data were expressed as . Single factor analysis of variance was used to compare the multiple sample means, and SNK method for group comparison.Changes in the cell growth curve were analyzed using repeated ANOVA.

Results

The mRNA and protein expression levels of ERβ1 and E-cadherin increased significantly in the transfection group compared with the control group (P<0.010). The vimentin mRNA level decreased significantly in the transfection group compared with the control group(P<0.010).The proliferation ability of the transfection group decreased either.

Conclusion

ERβ1 may play a role in inhibiting the epithelial-mesenchymal transition of breast cancer MDA-MB-231 cells.

Key words: Estrogen receptor β1, epithelial-mesenchymal transition, E-cadherin

Yan ZHOU, Li CHEN, Jia MING, Peng TANG, Yi ZHANG, Xin-hua YANG, Jun JIANG. Primary study on estrogen receptor β1 impeding epithelial-mesenchymal transition of breast cancer MDA-MB-231 cells[J]. Chinese Journal of Breast Disease(Electronic Edition), 2012, 06(04): 365-371.

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URL: https://zhrxbzz.cma-cmc.com.cn/EN/10.3877/cma. j. issn.1674-0807.2012.04.003

https://zhrxbzz.cma-cmc.com.cn/EN/Y2012/V06/I04/365

Figures/Tables 4

References 12

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组别	ERβ1	E-cadherin	Vimentin
未转染组	0.169±0.147	0.217±0.041	0.283±0.018
空载体组	0.182±0.045	0.213±0.044	0.287±0.039
pcDNA3.1-ERβ1转染组	0.479±0.081	0.412±0.041	0.149±0.010
F值	25.086	21.772	27.478
P值	0.001	0.002	0.001

组别	ERβ1	E-cadherin	Vimentin
未转染组	0.169±0.147	0.217±0.041	0.283±0.018
空载体组	0.182±0.045	0.213±0.044	0.287±0.039
pcDNA3.1-ERβ1转染组	0.479±0.081	0.412±0.041	0.149±0.010
F值	25.086	21.772	27.478
P值	0.001	0.002	0.001

组别	ERβ1	E-cadherin	Vimentin
未转染组	0.268±0.019	0.173±0.021	0.299±0.014
空载体组	0.289±0.025	0.185±0.021	0.302±0.012
pcDNA3.1-ERβ1转染组	0.714±0.096	0.360±0.014	0.280±0.014
F值	55.413	88.028	2.177
P值	0.000	0.000	0.192

组别	ERβ1	E-cadherin	Vimentin
未转染组	0.268±0.019	0.173±0.021	0.299±0.014
空载体组	0.289±0.025	0.185±0.021	0.302±0.012
pcDNA3.1-ERβ1转染组	0.714±0.096	0.360±0.014	0.280±0.014
F值	55.413	88.028	2.177
P值	0.000	0.000	0.192

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